INDICASAT AIP has a number of equipment that offers private and government companies, as part of its service portfolio.
Matrix Assisted Laser Desorption/Ionization double time-of-flight mass spectrometer (MALDI-TOF/TOF) ultrafleXtreme, Bruker: mass spectrometry is a technique used to measure the mass-to-charge (m/z) ratio of ions produced from analytes of interest. In this instrument the ionization facilitated using specific MALDI matrices (e.g. Sinapinic Acid, α-Cyano-4-hydroxycinnamic acid, etc.) that helps in transferring charge to analytes when irradiated and excite by a laser. MALDI allows setting experiments with wide range of selection, going from 500 Da to around 200,000 Da.
Bruker’s ultrafleXtreme has a resolving power around 40,000 and combined with fast acquisition rate (2 kHz speed in TOF mode and 1 kHz in TOF/TOF) allows flexible workflows from protein imaging, intact protein analysis, glycoproteomics, biologics or oligonucleotide quality control, to LC-MALDI proteomics. Latest TOF/TOF technologies allows high efficiency and sensitivity for peptide fragmentation studies (MS/MS) working with samples on the low fmol levels. Bruker’s ultrafleXtreme high resolution and rapid acquisition rate gives it the flexibility for a broad variety of complementary research, clinical and applied proteomics applications.
ElectroSpray Ionization / Atmospheric Pressure Chemical Ionization Quadrupole Time-of-Flight Mass Spectrometer (ESI/APCI-q-TOF) micrOTOF-QIII, Bruker: mass spectrometry is a technique used to measure the mass-to-charge (m/z) ratio of ions produced from analytes of interest. Currently we own two ionization source units for this equipment: an electrospray ionization (ESI) unit and an atmospheric pressure chemical ionization (APCI) which work by a facilitated ion formation when exposed to an electric field, heated inert desolvation gas (N2) and exposure to high voltages of a corona needle (in the case of APCI).
A resolution of >16.500 at FWHM (typically 18.000 FWHM) and a mass accuracy of <2ppm makes the micrOTOF-QIII the reference instrument in its class. Its resolving power and mass accuracy in combination with true isotopic pattern allows unambiguous sum formula determination or high resolved extracted ion chromatograms with <2mDa mass window in combination with any HPLC, UHPLC, CE and even GC. Molecular Formula Verification, De novo Formula Generation, Multi-Target Screening, Biomarker Discovery and Intact Protein Analysis are just a few application examples which can be covered by high performance TOF-MS systems.
Ultra High Performance Liquid Chromatographer (UHPLC) 1290 Infinity, Agilent Technologies: liquid chromatography is an essential tool in any chemistry related laboratory since it allows the separation of complex mixtures by exploiting the “small” chemical differences that exist among compounds. In the mass spectrometry lab it allows coupled LC-MS experiments where samples are previously separated before detection. This previous separation increases the window of vision of the available compounds.
The Agilent 1290 Infinity Binary LC is one of the most powerful and adaptive UHPLC system available. It can handle a wide range of applications: UHPLC methods, legacy HPLC methods, HPLC-to-UHPLC method transfer, method development, multi-compound screening, LC/MS coupling, and among others.
Fourier Transform Infrared Spectrometer (FTIR) ALPHA, Bruker: Fourier transform infrared spectroscopy (FTIR) is a technique which is used to obtain an infrared spectrum of absorption, emission, photoconductivity or Raman scattering of a solid, liquid or gas. An FTIR spectrometer simultaneously collects high spectral resolution data over a wide spectral range.
The ALPHA provides an optimal instrument design for almost each sample and therefore is greatly suitable for a large variety of applications. One of the most important applications of the ALPHA is quality control in various industries. Here the FTIR spectroscopic analysis is predominantly utilized to verify the identity and specifications of raw materials and products. Additionally the ALPHA allows the identification of unknown samples. This is particularly valuable for an effective failure analysis, e.g. when examining a contamination which caused a product defect. In criminalistics the possibility to determine unknown substances is applied to identify drugs, explosives and also trace evidence. Medical diagnostics uses the ALPHA to analyze kidney stones. The availability of liquid cells and various ATR options for the ALPHA allow performing quantitative analysis with always the optimal measurement conditions for the respective sample type.
Gas Chromatographs with Flame Ionization Detector, Nitrogen Phosphorus Detector, Electron Capture Detector, Thermal Conductivity Detector, and Mass Spectrometry Detector, Agilent Technologies: INDICASAT has gas chromatographs with different types of detectors that allows for the analysis of volatiles and semivolatiles compounds, and for the analysis of a diversity of compounds after derivatization.
The equipment can be used in analysis related with foods, oil, criminalistics, environmental samples, biomedical research, etc.